|
KMID : 0357920040380040228
|
|
Korean Journal of Pathology
2004 Volume.38 No. 4 p.228 ~ p.237
|
|
|
The Apoptotic Molecular Changes of Cellular Injury in Mouse Testis Induced by Endocrine Disrupting Chemicals
|
|
Wang Eun-Hui
Lee Kweon-Haeng
Jung Eun-Sun
Kang Chang-Suk
Choi Yeong-Jin
Yang Ki-Hwa
Lee Jin-Suk
|
|
|
Abstract
|
|
|
Background: Spermatogenesis is regulated by various cellular reactions, and especially cell proliferation and apoptosis.
Methods: We investigated the morphological changes and the apoptotic molecular changes in mouse testis induced by the endocrine disrupting chemicals. ICR mice were treated with bisphenol A (BPA), 2-bromopropane (2-BP) and diethylstilbesterol (DES). Histological examination and immunohistochemical staining, TUNNEL staining and RNAse protection assay were conducted.
Results: Testes treated with BPA showed normal spermatogenesis and the proliferation activity, and the density of the cells was similar with those in the control. 2-BP and DES groups, which showed a decrease of germ cells near the basal layer and degenerative changes. The proliferative activity identified by PCNA staining was significantly decreased in the 2-BP and DES groups (p<0.05). The apoptosis was significantly increased in the 2-BP group however, a significant decrease was noted in the BPA group (p<0.05). Among apoptosis-related molecules, the expression of Fas, Fas ligand, TRAIL, TNFp55 and caspase 1, 3, 6 and 8 were changed according to the change of the degree of apoptosis in all groups.
Conclusions: Endocrine disrupting chemicals induced cellular injury in mouse testis through the changes of proliferative activity and apoptosis which was regulated by a number of apoptosis-related molecules. This probably results in the abnormality of spermatogenesis in mouse testis.
|
|
|
KEYWORD
|
|
|
Bisphenol A, 2-Bromopropane, Diethylstilbesterol, Spermatogenesis, Testis
|
|
|
FullTexts / Linksout information
|
|
 
|
|
|
Listed journal information
|
|
  
|
|